Abstract

Reactive species or free radicals include reactive oxygen and nitrogen species that are called reactive oxygen nitrogen species. Reactive oxygen species are formed as a natural by-product of the normal metabolism of oxygen and have significant roles in cell signaling and homeostasis. The reactive oxygen species are generated as a by-product of biochemical reactions, in mitochondria, peroxisomes, cytochrome P450, and other cellular components. When oxygen homeostasis is not maintained, oxidative stress is increased in the cellular environment. Superoxide, hydrogen peroxide and hydroxyl radicals are normal metabolic by-products which are generated continuously by the mitochondria in growing cells. Microsomal cytochrome P450 enzymes, flavoprotein oxidases and peroxisomal enzymes are other significant intracellular sources of reactive oxygen species.

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