Abstract
A few yeasts are able to utilize methanol as a sole carbon and energy source. The metabolism of methanol has been studied in detail in Hansenula polymorpha and Candida boidinii. The enzyme involved in the metabolism of methanol is methanol or alcohol oxidase (EC 1.1.3.13). It converts methanol to formaldehyde in the presence of molecular oxygen. The co-product, hydrogen peroxide, can be dismutated to O2 and H2O in the presence of catalase which is intimately associated with alcohol oxidase in the cell. Formaldehyde in turn is dissimulated to CO2 by formaldehyde dehydrogenase and formate dehydrogenase and, perhaps, by catalase.
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