Biochemical Traits in the Flower Lifetime of a Mexican Mistletoe Parasitizing Mesquite Biomass.

Elizabeth Quintana-Rodríguez,Xicotencatl Camacho-Coronel,José Esparza-Claudio,Domancar Orona-Tamayo,Alan Gamaliel Ramírez-Rodríguez,Jorge Molina-Torres,Martin Heil,Enrique Ramírez-Chávez

doi:10.3389/fpls.2018.01031

Elizabeth Quintana-Rodríguez, Xicotencatl Camacho-Coronel + Show 6 more

Open Access

https://doi.org/10.3389/fpls.2018.01031

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Abstract

Psittacanthus calyculatus is a hemiparasitic plant that infects a wide range of trees. Mainly the biology reproduction of this mistletoe lies in bright colored flower development. Furthermore, it uses the nectar secretion as the only reward to engage different flower visitors. We investigated the physiological mechanisms of the flower phenology per hour and per day to analyze the spatial-temporal patterns of the nectar secretion, Cell Wall Invertase Activity (key enzyme in the quality of nectar), nectar chemistry, volatile organic compounds (VOCs) emission, synthesis of carotenoids and frequency of floral visitors. Flowers lasted 4 days, total nectar was loaded just before the anthesis and the secretion was maintained over day 1 and 2, decreased on day 3, and stopped on day 4. The diurnal nectar secretion dynamic per hour on day 1 and 2 showed similar patterns with high production on the morning and a decrease in the afternoon, the secretion declined on day 3 and ceased on day 4. On the other hand, CWIN activity per day was less before the anthesis and increased on day 1 and 2, this enzymatic activity decreased on the old flower phenology. Moreover, diurnal CWIN activities showed different patterns in the morning, noon, and lastly in the afternoon. Nectar chemistry varied significantly throughout of the flower lifetime, sucrose decreased along the flower phenology increasing glucose and fructose. Amino acids showed the prevalence of proline and oxo-proline, both increased on the day 1 and diminished in subsequent old flower stages. The spatial VOCs emission showed the presence of 11 compounds being β-ocimene the main volatile; its release increased on day 1 and remained constant in the flower lifetime. Lutein, lycopene, and β-carotene were concentrated in old stages of the flowers. In field, the most frequent flower visitors were the hummingbirds that usually foraging in all phenologic flower stage and their foraging events decreased with the phenological flower lifetimes. The results showed that these traits presented by P. calyculatus flowers are able to engage and manipulate the behavior of flower visitors and contribute to the reproduction of the parasitic plant.

Highlights

The attraction of pollinators by flowers is based on different traits such as: floral nectar (FN) secretion, volatile organic compounds (VOCs) emission, and the production of color compounds in the flowers, these features are called pollination syndromes (Knudsen and Tollsten, 1993; Faegri and Van der Pijl, 2013)
The experiments were performed in a population of Prosopis laevigata highly infected with P. calyculatus located in a suburban area near of Irapuato in the state of Guanajuato in Central Mexico (20◦43 N; 101◦19 O at 1,730 m a.s.l)
On day 0” (D0), buds were partially open on the tip and presented a light yellow coloration, color presented on day 1 (D1), this color persisted on day 2 (D2), while on day 3 (D3) through day 4 (D4) petals turned on a bright orange and on the day 5 (D5) the petals fallen completely

Summary

Introduction

The attraction of pollinators by flowers is based on different traits such as: floral nectar (FN) secretion, volatile organic compounds (VOCs) emission, and the production of color compounds in the flowers, these features are called pollination syndromes (Knudsen and Tollsten, 1993; Faegri and Van der Pijl, 2013). They are present in a variety of forms and are exclusively found in the tropical native species of South and Central America, Africa, Australia, and New Zealand (Fadini et al, 2018) These plants usually present different traits to manipulate the pollinator behavior that include: extended flowering lifetime (Azpeitia and Lara, 2006), production of high quality FN (Rivera et al, 1996; Pérez-Crespo et al, 2016), VOCs emission (Bungert et al, 2002; Sipes et al, 2014) and the developments of bright colored flowers (Pérez-Crespo et al, 2016). Among mistletoes the Loranthaceae family is one of the largest and the most diverse (73 genera and ∼990 species) (Nickrent et al, 2010)

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