Biochemical study on long non coding RNA gene expression in women having preeclampsia

Abstract

BackgroundPreeclampsia (PE) is a familiar gestational complication that affects 3% to 5% of pregnant women. After 20 weeks of pregnancy, PE typically presents as new-onset hypertension and proteinuria. Inadequate trophoblast invasion, accompanied by defects in placental vasculature formation and subsequent placental underperfusion, has been linked to the pathology of PE. Via changes in the biological functions of trophoblasts, lncRNAs may influence the occurrence and progression of PE. ObjectivesThe objective of this research is to see whether the levels of expression of Metastasis-associated lung adenocarcinoma transcript1 (MALAT1) and SPRY4 intronic transcript 1 (SPRY4-IT1) are linked to preeclampsia susceptibility and pathogenesis. Subjects and methodsA total of 195 people took part in this report. A hundred and thirty were patients with preeclampsia (65 mild and 65 severe Preeclampsia). The control group contained 65 normally pregnant women. Both groups had similar age. All groups were subjected to (complete history taking focusing on age, parity and past history of hypertension or diabetes mellitus) thorough clinical examination. The Determination of MALAT1 and SPRY4-IT1 expression levels in placental tissue have been performed by real time q PCR technique. Results showedMALAT1 and SPRY4-IT1 expression levels were found to vary significantly between the studied groups, with lower MALAT1 and higher SPRY4-IT1 expression levels in Preeclampsia patients than in controls. ConclusionFrom this study, it could be concluded that Malat1 and SPRY4-IT1 expression levels might be used as predicting markers for pathogenesis and diagnosis of preeclampsia.