Biochemical study of some non invasive markers in liver fibrosis patients.

Abstract

Non-invasive methods have been proposed as surrogate markers for liver biopsy. It was shown that serum hyaluronic acid (HA) level increase with the development of liver fibrosis. The present study aims to determine serum HA level cut-off points for predicting liver fibrosis. Serum HA level in chronic hepatitis patients (n=60) are divided into two groups, group1: included 30 patients, there were positive for anti-HCV (antibodies), group2: included 30 patients, there were positive for HBsAg, and controls (n=10) were assessed by ELISA and liver histopathological parameters were evaluated by the modified Knodell score and microscopic examination of liver biopsies. The results showed that individuals in healthy control group have normal levels of HA (mean 14.3, SD: 5.5) while the levels of HA were elevated in patients of HCV alone (mean 103.6±28.0) and in patient of HCV (mean 104.5± 37.5).Also levels of HA were poorly elevated in HBV alone (mean 62.2± 15.5) and in HBV (mean 45.8± 12.4).The comparison between the studied groups regarding to the level of HA showed that a significant difference was observed between healthy group and HBV & HCV groups (P < 0.001).HA levels and stages of fibrosis were well correlated in patients of HBV and HCV group. Where, this is a significant increase in HA levels when considering F0 to F6 scores by liver biopsy (P < 0.001). Serum HA levels are well correlated with HAI In patients Of HBV & HCV groups where, there was significant increase in HA levels by increase of HAI in liver biopsy at P < 0.001.