Biochemical Studies on Evaluation of New Composite against Hepatocellular carcinoma-induced in Rats: Recent Therapeutic Approaches

Abstract

The hepatoprotective effect of some natural and synthetic compounds against chemically inducedhepatocellular carcinoma in rats was evaluated. One hundred male albino rats were divided equally intofive groups. Normal control group, carcinogenic [Ferric nitrilotriacetate (Fe-NTA: 9 mg Fe/kg b.wt. i.p.)and chloroform (150 mg/ kg b.wt. orally)] - induced group, curcumin group (400 mg/kg. b.wt. orally),tetrachlorocuprate-lysine (25 mg/kg. b. wt. s.c.) and ascorbate (500 mg/kg. b. wt. orally) group and amixture group (composed of curcumin, tetrachlorocuprate -lysine and ascorbate). Blood samples andliver tissue specimens were collected at the end of experiment (4 months) for determination of thefollowing parameters: Tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), catalase andmyeloperoxidase (MPO) in liver tissues, in addition to serum malondialdehyde (MDA) and reducedglutathione (GSH). Moreover, histopathological examination of liver tissues was done for resultsconfirmation. The obtained results showed a significant elevation in MDA, MPO and immunologicalmarkers levels, with significant reduction in serum reduced glutathione and catalase activity in livertissue in hepatocellular-carcinogen induced rats as compared to the control group. However,administration of rats with the compounds under investigation resulted in a significant reduction ofMDA, MPO and immunological markers levels, and increased in reduced glutathione and catalase levelscompared to the carcinogenic non treated group. Various pathological alterations were observed in liverof chemically induced-carcinogenic group interestingly, results supported the protective effect of thecompounds under investigation and preserved the histological structures of liver tissues. These resultsconcluded that basic curcumin, tetrachlorocuprate-lysine and ascorbate exert chemopreventative effectagainst hepatocellular carcinoma.