Abstract

In the current experiment, the conversion of dl-norleucine and dl-alanine to their respective α-keto analogue by Piricularia oryzae was confirmed by the separation of 2,4-dinitrophenylhydrazone of either keto acid. Although the formation of keto acid from norleucine was distinctly recognized both in the presence and the absence of arsenite, the accumulation of pyruvic acid from alanine was only found in the presence of arsenite, as it has been observed with some microorganisms. In addition, the preferential utilization of alanine to a proper carbon source was identified, which may provide a step towards the investigation of the nutritional relationship between the blast fungus and the host plant.

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