Abstract
The objective of this study is to elucidate the effect of Pirimiphos-methyl (Organophosphorus insecticide) on protein profile of insects that found in/above or around treated rabbit (Oryctolagus cunicullus domesticus L.) carrions. Additionally, an esterase analysis was performed to clarify the effect of this insecticide on necrophagous insects. Biochemical studies were carried out on collected adult Dermestes maculatus De-Geer and pupae of Chrysomya albiceps (Weidemann) for their forensic importance. Four protein fractions observed only in the treated beetles and one unique band for the control group. Treated Chrysomya albiceps pupae exhibited 14 specific bands whereas two bands were only exhibited by the control. Genetic distances calculated for treated and control insects were 0.38 and 0.42 for adult Dermestes maculatus and pupae of Chrysomya albiceps, respectively. Variations in protein bands may be interpreted that some pesticides enhance the transcription of certain sequences which are probably related to resistance and /or detoxification mechanisms. Esterase pattern analysis using α-naphthyl acetate reflected 4 characteristic bands for treated beetles. However, using β-naphthyl acetate yielded 4 specific bands for the control group. For Chrysomya albiceps pupae, conduction of α- naphthyl acetate yielded one common band for both treated and control groups and one specific band for the control group. Whereas, using of β- naphthyl acetate substrate yielded two common bands shared by both groups and one specific band for the control group. Forensically important insects such as Chrysomya albiceps Weidemann and Dermestes maculatus De-Geer have an active esterase system.
Highlights
One unique band was absent in the treated beetles profile
This figure was similar to the Chrysomya pupae profile as well, since pupae from the treated group exhibited 14 unique bands whereas two bands exhibited by control ones were lacking in the treated pupae profile
Variations in protein bands, may be explained on the basis that some pesticides enhance the transcription of certain sequences which are probably related to resistance and /or detoxification mechanisms (Salam et al, 1984)
Summary
Victims are not discovered for many days to months and because of it, entomological techniques are useful in forensic analysis (Carvalho et al, 2001). Deaths as a result of OP poisoning are usually detected by analysis of body fluids and tissues for the presence of the toxic agent. In the first few hours, a pathologist can provide a reasonable estimate of Post mortem interval (PMI) from the physical and histochemical consequences of death; which represent particular difficulties when performed on remains in an advanced stage of decomposition (Gunatilake et al, 1989). Study of nechrophagous insects can be contributed to the qualitative identification of substances or abuse drugs. Researches have shown that it is possible to detect chemical substances even in pupariae. Researches have shown that it is possible to detect chemical substances even in pupariae. (Carvalho et al, 2001)
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