Abstract
Zinc is an effective anti-inflammatory and antioxidant trace element. The aim of this study was to analyse the protective effect of zinc and zinc–prolactin systems as additives of preservation solutions in the prevention of nephron damage caused during ischemia. The study used a model for storing isolated porcine kidneys in Biolasol®. The solution was modified with the addition of Zn at a dose of 1 µg/L and Zn: 1 µg/L with prolactin (PRL): 0.1 µg/L. After 2 h and 48 h of storage, the levels of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, sodium, potassium, creatinine and total protein were determined. Zinc added to the Biolasol® composition at a dose of 1 µg/L showed minor effectiveness in the protection of nephrons. In turn, Zn2+ added to Biolasol + PRL (PRL: 0.1 µg/L) acted as a prolactin inhibitor. We do not recommend the addition of Zn(II) (1 µg/L) and Zn(II) (1 µg/L) + PRL (0.1 µg/L) to the Biolasol solution.
Highlights
Zinc is a trace element that regulates many biochemical processes in the human body.It affects proper development, participates in the synthesis of hormones, is a cofactor of active thymulin (ZnFTS, zinc-facteur-timique-serique) released by thymic cells, and participates in the stabilization of cell membranes
Zinc participates in the regulation of intra- and extracellular pathways involved in cell proliferation, differentiation, development, apoptosis, and transformation [8]
Like Brandão et al [41], that zinc is an inhibitor of prolactin, reduces its activity and removes its protective effect
Summary
Zinc is a trace element that regulates many biochemical processes in the human body. It affects proper development, participates in the synthesis of hormones (testosterone, insulin), is a cofactor of active thymulin (ZnFTS, zinc-facteur-timique-serique) released by thymic cells, and participates in the stabilization of cell membranes. Cierpka’s team [11] compared the effectiveness of Biolasol and UW in the animal model of perfusion and preservation of the isolated kidney of the Polish Large White pig They analysed changes in the concentration of enzyme markers (ALT, AST, LDH) in the collected perfundates, specimens collected for histopathological examinations and mean survival time of the recipient after autotransplantation. The addition of 1 μg/L Zn2+ to the modified Biolasol + PRL solution (group B3) caused a significant increase in the activity of enzymes in the kidney homogenates. We found a minor protective effect of zinc added to Biolasol at a dose of 1 μg/L on nephrons This is probably because the high intracellular Zn2+ concentration induces its pro-oxidative properties and influences the conditions of oxidative stress [43]. More research is essential to elucidating the role relating to zinc (his dose) in the efficiency of preservative solution
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
