Biochemical studies during the development of the chick embryo. VI. On a deoxyribosyl compound in the ribonucleic acid preparation.

Abstract

Ribonucleic acid prepared according to modified Kirby’s phenol method from 14 day old chick embryos was fractionated into three peaks by MAK column chromatography. To the peak eluted in the range from 0.65 to 0.7 m NaCl, the name “Peak II” was given tentatively. Purified Peak II was homogeneous upon ultracentrifugation, polyacrylamide gel electrophoresis and elution patterns of chromatographies.The peak gave a positive result with the orcinol reaction. Furthermore, the hydrolyzate by DNase and snake venom was active in promoting the growth of L. leichmannii 7830. The peak was not disrupted by treatment with RNase, but was with DNase. Peak II treated with heat gave the same fractionation profile as the native peak by gel filtration on Sephadex G–200.By treatment with 0.3 n KOH at 37°C for 18 hr, Peak II was resolved into two peaks, Peak II–1 and Peak II–2. It was found that Peak II consisted of the susceptible and the non-susceptible components to the alkali. From the susceptible component (Peak II–2) the ...