Biochemical properties and usefulness of boar semen for liquid preservation following atropine administration.

Abstract

Four sexually mature boars were used in two different experiments. In Experiment I, two boars were injected once subcutaneously with atropine and, ejaculates were collected after 30, 60 and 90 min. In Experiment II, each boar was injected weekly with 25, 37.5 and 50 mg of atropine, and ejaculates were collected after 30 min. Ejaculates obtained in Exp. II were diluted with Kortowo-3 extender (Olsztyn, Poland), with and without the addition of low density lipoprotein fraction (LDF) isolated from hen egg yolk,and stored at 5 degrees C and 16 degrees C for 5-6 days. Atropine caused a decrease in semen volume and an increase in sperm concentration with fewer agglutinated spermatozoa. Changes in sperm motility were not significant. There was a significant increase in the content of fructose, zinc, citric acid and protein in the seminal plasma of atropine-injected boars. Furthermore, increased antiproteolytic and antiperoxidant activity as well as seminal phosphatases were also observed. No significant changes were observed in the content of free sialic acid, whereas bound sialic acid was significantly increased in Exp. II. A decrease in osmolality and pH of seminal plasma was observed. Electrophoretic studies revealed that there were alterations in the molecular forms of seminal phosphatases and proteinase inhibitors. There were no significant changes in the percentage of morphologically abnormal spermatozoa, osmotic plasmalemma resistance at the acrosomal region (ORT) and malondialdehyde production in the spermatozoa. AspAT activity recovered from cold shocked spermatozoa was significantly reduced, whereas disturbances in plasma membrane permeability to fluorochrome HO 258 were observed in Exp. I. Semen of atropine-injected boars had increased sperm viability during liquid preservation at 5 and 16 degrees C.