Abstract
Biochemical potential and screening of bioactive components of Ocimum basilicum
Highlights
Introduction capacity ofLamiaceae family plants, theyPlants provide us with many important are widely used by mankind
Current study was planned to investigate the biochemical potential of sweet basil (Ocimum basilicum) along with the screening of some important phytochemicals through biological assays and other analytical techniques
The most imperative part of our research work was the separation of phenolic acids through High performance liquid chromatography (HPLC) and analyzing crude basil powder by using the FTIR technique. 2, 2-diphenyl-1-1-picrylhydrazylstable radical (DPPH) scavenging assay diphenyl-1-1picrylhydrazylstable radical (DPPH) is a constant free radical usually used to find out the capability of compounds to scavenge free radicals
Summary
Liquid Chromatography (HPLC). 500 mg dried leaf powder was ground in particle size of 0.125–0.250 mm. immersed in 5 ml of methanol of HPLC grade and left at 4 °C overnight in darkness. 500 mg dried leaf powder was ground in particle size of 0.125–0.250 mm. Immersed in 5 ml of methanol of HPLC grade and left at 4 °C overnight in darkness. Decantation and filtration of supernatants was done through syringe filter [17] and samples were shifted to HPLC vials. FT-IR assay was executed on crude basil powder sample to identify its functional groups. Infrared spectral studies were carried out on FT-IR spectrometer in the presence of dry air, and it was performed at room temperature. In Potassium bromide (KBr) on the FT-IR spectrophotometer using KBr pellet, the spectra from the samples were measured [18]
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