Abstract

The effect of sub-lethal concentrations (1.88, 3.75, 7.50, 15.00 and 30.00 mg/L) of crude leaf extract of Cannabis sativa was determined in the plasma, liver and gill biochemical parameters of Common carp, Cyprinus carpio (mean weight of 15.05±0.05g) after 56-day exposure period in static renewable bioassay system. During the experiment, some physicochemical parameters were monitored while at the end of the experiment, the selected biochemical parameters were determined in the plasma, liver and gill of the test fish. The biochemical parameters determined were alkaline phosphatase (ALP), Lactate dehydrogenase (LDH), total protein (TP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), bilirubin, total bilirubin (TB), albumin (ALB), urea acid (UA) and cholesterol (CHOL). The monitored pH showed significant difference (p 0.05) in C. sativa test set-ups compared to the control during the experimental period. There were significant difference (p<0.05) in some of the determined biochemical parameters of the test fish exposed to C. sativa compared to the control. Therefore, it can be deduce from the study that prolonged exposure of C. carpio fingerlings to crude leaf extract of C. sativa affected some determined biochemical parameters in the tissue/organs of the test fish.

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