Abstract
Using mediator as electron acceptor for biochemical oxygen demand (BOD) measurement was developed in the last decade (BODMed). However, until now, no BODMed in a flow system has been reported. This work for the first time describes a flow system of BODMed method (BODMed-FS) by using potassium ferricyanide as mediator and carbon fiber felt as substrate material for microbial immobilization. The system can determine the BOD value within 30min and possesses a wider analytical linear range for measuring glucose–glutamic acid (GGA) standard solution from 2 up to 200mgL−1 without the need of dilution. The analytical performance of the BODMed-FS is comparable or better than that of the previously reported BODMed method, especially its superior long-term stability up to 2 months under continuous operation. Moreover, the BODMed-FS has same determination accuracy with the conventional BOD5 method by measuring real samples from a local wastewater treatment plant (WWTP).
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