Biochemical Investigation of Goat Milk Casein and Whey Protein Crude Methanol Extract.

Abstract

Milk and milk products is implicated in many human health benefits. Several animals milk source are used for that porous such as cow, cheap, camel and goat. Goat milk has a unique composition compared to other animals. In this study, we investigate the antioxidant, antibacterial and anticancer potencies of casein and whey proteins of goat milk fractions crude methanol extract. As a result, whey protein extract exhibited high antioxidant activity than casein against stable radical DPPH and ABTS with IC50 value 0.12 and 0.237 µg/ml, respectively compared to casein fraction extract. The anticancer properties were investigated against two cancerous cell lines, including: breast (MCF-7) and liver (HepG2). Results of the MTT assay were in agreement of the antioxidants activity, when whey fraction methanol extract exhibited specific anticancer activities against MCF-7 cell line compared to casein and doxorubicin positive control. However, no cytotoxic activities of extract were observed against two non-cancerous control cell lines (BJ-1and MCF-12). Casein and whey proteins extract could prevent DNA damage induced by oxidative stress. The antimicrobial activity of goat milk methanol extract was tested against five bacterial strains including gram positive (Bacillus cereu EMCC 1080 and Staphylococcus ureuse ATCC 13565(, gram negative (Escherichia coli O157-H7 ATCC 51659, Salmonella typhi ATCC 15566 and Pseudomonas aeruginosa NRRL B-272. Among them, Bacillus cereu and Escherichia coli were extensively affected, when the inhibition zone was 4.3 and 5 cm, respectively. The fact that goat milk is enriched in features belonging to elements, vitamins and short peptide derivatives. In addition, goat milk has specific fat composition of the medium chain fatty acids led to increase the antioxidant and antibacterial and anticancer properties.