Year-end Sale % OFF 
Abstract
The environmental metal cadmium is a carcinogen that has been shown to increase the rate of mutations by inhibiting DNA mismatch repair, and to replace zinc ions within the active site of certain endonucleases. Here, the mutagenic property of cadmium was further explored with a model DNA polymerase, Thermus aquaticus DNA Polymerase I (Taq Pol I). Diminished efficiency of Taq Pol I was observed as a function of cadmium concentration. Using a series of polymerase chain reactions (PCR) and circular dichroism spectroscopic assays with full-length Taq Pol I, structural changes in the presence of low cadmium concentration was a significant factor in the observed change in enzyme efficiency. These structural changes provide new insights into the mutagenic property of cadmium.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
