Abstract
We investigated the effects of decreasing phenoloxidase (PO) activity and prophenoloxidase ( proPO) gene expression on the inhibition of postharvest melanosis formation in the red queen crab, Chionoecetes japonicus. The cDNA of proPO from hemocytes of C. japonicus was partially cloned and sequenced. Immersion of live crabs in a 1.0% ergothioneine (ESH)-rich mushroom extract ( Flammulina velutipes; ME) solution resulted in significant inhibition of haemolymph PO activity and a reduction of the proPO gene expression in hemocytes that consequently controlled melanosis in the crabs during ice storage. Treatments with a 0.05% w/v sodium sulphite solution or a 0.05% w/v 4-hexyl-1,3-benzenediol solution had similar positive effects as the treatment with a 1.0% ME solution in vivo. In vitro experiments showed that authentic l-(+)-ESH inhibited PO activity and decreased proPO gene expression in crab hemocytes. Thus, the application of ESH-rich ME can be a novel alternative to synthetic melanosis-inhibiting agents to control postharvest melanosis in crabs.
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