Biochemical indices of oxidative stress and inflammation in the evaluation of peripheral artery disease

Abstract

BackgroundThe aims of this study were: (1) to investigate changes in indices of oxidative stress and inflammation in the evaluation of peripheral artery disease (PAD); (2) to compare the diagnostic efficacy of these parameters with that of classical clinical laboratory routine parameters. Design and methodsWe studied 115 patients with PAD and 300 healthy volunteers. ResultsPAD patients had significantly increased circulating concentrations of F2-isoprostanes, protein carbonyls, chemokine (C-C motif) ligand 2 (CCL2), high-sensitivity C-reactive protein (hs-CRP), β-2-microglobulin (B2M), and decreased paraoxonase-1 (PON1) levels. When patients were classified according to the Fontaine score, we observed important increases in plasma F2-isoprostanes and CCL2 that appeared in milder stages of the disease, and remained so at similar levels in more advanced stages; almost no overlapping with the control group was noted. Receiver operating characteristics analysis comparing patients and controls revealed that the areas under the curve for F2-isoprostanes and CCL2 approached unity [0.999 (0.998–1.000) and 0.993 (0.985–1.000)], respectively, and significantly higher to those of the other measured parameters. ConclusionOur data suggest that F2-isoprostanes and CCL2 measurements may be useful tools for the diagnosis of PAD.