Abstract
The lysine plus threonine (LT) resistant maize mutant Ltr ∗1 selected by culturing maize cells in the presence of lysine plus threonine [1] was transferred by repeated back-crosses to maize inbred lines containing normal, britle (bt), shrunken-2 (sh2) and opaque-2 (o>2) endosperms. Genetic analysis located the Ltr ∗1 gene on the short arm of chromosome 7 at 10.6 centimorgans from the o2 gene. The presence of the Ltr ∗1 gene increased the level of soluble threonine in the normal endosperm by 8–18-fold. A synergistic effect on the increase of soluble threonine was observed when the Ltr ∗1 gene was combined with endosperm mutations. An increase of 45–144-fold in soluble threonine and 3–10-fold in total soluble amino acid pool was observed in the double mutant Ltr ∗1Ltr ∗1/o2o2 when compared with o2 and normal endosperms, respectively. In general it was observed that the Ltr ∗1 gene intensified the effect of the o2 gene on amino acid and protein synthesis in maize endosperm.
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