Abstract
ABSTRACT In vivo and in vitro metabolic studies were conducted on the effects of the insect growth regulator diflubenzuron (DFB) on chitin synthesis in the postmolt blue crab Callinectes sapidus. The effects of the pesticide on the incorporation of either 3H-glucosamine or 3H-N-acetylglucosamine into sodium dodecyl sulfate (SDS) insoluble cuticular residue were examined. The radiolabeled product formed was identified as chitin by chemical and enzymatic criteria. 3HN-acetylglucosamine was found to be incorporated to a greater extent than 3H-glucosamine during metabolic studies. During in vitro explant culture experiments, the highest concentration of DFB tested (1 ppm) caused 98% inhibition of chitin synthesis; 64% inhibition was observed at concentrations as low as 0.7 parts per billion. The results indicate that diflubenzuron inhibits the incorporation of 3H-N-acetylglucosamine into cuticular chitin in postmolt blue crabs. The data are consistent with our previous autoradiographic and ultrastructural studies on the effects of DFB in the blue crab; i.e., that the Golgi complex is disrupted by DFB treatment.
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