Biochemical Characterization of Ridge Gourd and Sponge Gourd Genotypes

Abstract

A genetic analysis of ridge gourd and sponge gourd genotypes were performed using protein profiling and isozymes analysis of four enzymes viz., peroxidase, polyphenol oxidase, esterase and super oxide dismutase. Fifteen day old seedlings of the genotypes were tested in Native-PAGE for the study of genetic diversity among them. Protein profiling of the genotypes developed nine bands having Rm values of 0.037, 0.075, 0.154, 0.213, 0.266, 0.326, 0.532, 0.584 and 0.663. Out of them, three bands were monomorphic and others were polymorphic. Seventeen ridge gourd and sponge gourd genotypes were grouped into two main clusters I and II with an average similarity of 47 per cent. Four bands of peroxidase isozymes were observed having Rm values of 0.083, 0.236, 0.515 and 0.803. Out of them two bands were polymorphic in nature. Four bands of polyphenol oxidase were detected with Rm values of 0.318, 0.438, 0.570 and 0.686. Out of them two bands were polymorphic in nature. Total five bands of esterase were observed having Rm values of 0.037, 0.102, 0.170, 0.269 and 0.372. Out of them four bands were polymorphic in nature and a band with Rm value 0.102 was monomorphic. Three bands of Rm values 0.246, 0.339 and 0.486 were observed for super oxide dismutase isozymes. The band of Rm value 0.486 was present in all the 17 genotypes and was monomorphic in nature.