Abstract
Abstract. The rate of reduction of ferrihemoglobin within the erythrocytes and the oxygen affinity of red cells have been measured as a function of time of blood storage. The efficiency of the ferrihemoglobin‐reducing systems decreases with time of storage as measured both in presence of glucose or lactate as substrates. The well‐known increase with ageing of the oxygen affinity (as measured by p1/2) for blood stored in ACD is observed when the p1/2 is determined in Tris‐HCl buffer and parallels the decay of the rate of ferrihemoglobin reduction. However, the increase in oxygen affinity is not observed when the p1/2 is measured in phosphate buffer; this is explained as being due to the replacement of the originally hemoglobin‐bound DPG by phosphate ions entering the red cell.
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