Abstract
Biochemical changes in the serum and liver of albino rats chronically exposed to rats administered 5gk-1 , 7.5gk-1 and 15gk-1 of gasoline , kerosine and crude petroleum(bonny light) respectively were studied. The petroleum samples were administered intraperitoneally and the biochemical changes in the rat serum and the liver wree monitored over a three monthduration:Asparate amino transferase (AST) Alanine amino transferas (ALT) and Alkaline Phosphate (ALP) all showed dose- dependent increase in levels from zero month to 3rd month, gasoline from 19.0 ± 2.8 of control to 69.0 ±12.8 third month kerosine 19.0 ±2.8 control to 58±5.0 third month and crude Petroleum 19.0±2.8 control to 33.2 ± 1.3 at the third month. Alanine amino transferase increase from 12.0 ± 1.1 of control to 62.0 ± 6.0 at the third month with gasoline, also from 12+ 1.1 of control to 29.7 + 5.6 in the third month with kerosene and from 12.0 ± 1.1 of control to 27. ± 3.1 at the third month with crude petroleum. Furthermore, the alkaline phosphatase increase from 62.1 ± 3.0 ì/l control to 161.0 ± 2.0 at the third month for gasoline, kerosene from 62.1 ± 3.0 of control to 123.6 ± 12.6ì/l at the third month, with crude petroleum (bonny light) increasing from 62.1 ± 3.0 ì/l of control to 90.5± 6.3 ì/l at third month. Glutathione transferase (GST) was marginally increase in gasoline treated rats from 13.0 ± 0.8 ì/l of control to 17.0 ± 1.0 u/l however, greater elevation in level of the enzyme was obtained in kerosene treated rats from 13.0 ± 0.8 ì/l of control to 25.2 ± 2.0 ì/l at the third month. Finally the reducedglutathione (GSH) seemed to be depleted from 0.56 ± 0.1 mM of control to 0.43 ± 0.1 at the third month with gasoline, 0.30 ± 0.1 mM at the third month with kerosene and 0.41 ± 0.1 mM at the third month with crude petroleum (bonny light). In conclusion the petroleum sample caused biochemical changes in the serum andliver of the rats.
Highlights
The liver contains numerous enzymes some of which are present in serum in very low concentration
The aim of the current study is to study the biochemical changes that occurs in the serum and liver of albino rats exposed to petroleum samples
Serum activities of Aspartate transaminase (AST)< Alanine Transaminase (ALT)< and Alkaline Phosphate (ALP) showed a significant elevation from the first month of exposure till the end of three months study
Summary
The liver contains numerous enzymes some of which are present in serum in very low concentration. These enzymes have no known function in serum other than to provide information about hepatic state and disorders.Reports of Kaplowitz, 1992; Dede, 1992 indicated that elevated serum transferase and alkaline phosphatase levels as indicators of liver necrosis. The aim of the current study is to study the biochemical changes that occurs in the serum (enzymelevels) and liver of albino rats exposed to petroleum samples (gasoline, kerosene and crude petroleum (bonny light)
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