Abstract
Singh A., P. P. Gupta, S. P. Ahuja, H. S. Banga: Biochemical Changes in Milk in Experimental Acholeplasmal Mastitis in Goats. Acta vet. Brno, 60, 1991: 193-203. To study the biochemical changes in milk during unilateral acholeplasmal mastitis, 8 lactating goats were selected, out of which 7 animals were inoculated through the right teat canal with 2 ml of Acholeplasma laidlawii containing 109 colony forming units per 1 ml. The left udder halves of these 7 goats served as controls and one goat was kept as intact control. The effects of infection were monitored over a period of 19 days. During this period one goat was killed every 3rd day after infection, while 2 animals were killed on the 19th day, i.e. one experimentally infected and one intact control. All the goats infected with A. laidlawii developed clinical mastitis within 24 h, which persisted till the end of the experiment. The total protein, total immunoglo bulins (Ig), total phospholipids and free fatty acid contents of mastitic milk/mam mary secretions showed progressive increase, whereas total lipids and glycerides decreased substantially. However, there was a marginal increase in the total choleste rol concentration. The results indicated that Acholeplasma use lipolytic enzymes to degrade lipids so as to meet their needs of energy and for biosynthesis of membrane lipid bilayer during multiplication. The increase in milk protein especially Ig indicated humoral immune response to A. laidlawii and secretion of Ig into the mammary gland. Goats, milk/secretion, total proteins, lipids, cholesterol, FFA, phospholipids, glycerides, immunoglobulines
Highlights
To study the biochemical changes in milk during experimental mycoplasmal mastitis, 11 lactating goats were taken fld 2 ml of Mycoplasma mycoides subsp. capri culture containing 10 colony foming units per ml wer~ inoculated through the teat canal into their right udder halves
The right halves of the udder of all the 11 goats inoculated with M. mycoides subsp. capri, became, hot, tender and painful 2 days after being inoculated
The re-isolation of M.:.. mycoides subsp. capri from the mammary secretions even on the 18th day post-inoculation confirmed that the mastitis was of mycoplasmal origin
Summary
Eleven· lactating goats aged from 2,5 to 4 years were kept under observation for 7 days before starting the experiment. The left halves of udders of these goats were inoculated with 2 ml of sterile mycoplasma broth and served as contro. Pre and postinoculation samples of the milk/mammary secretions were obtained from all the goats on third, seventh, eighteenth and twenty-fifth day after inoculation and were cultured on mycoplasma medium Total leucocyte counts (TLC) of milk/mammary secretions and haematological values of all the goats were recorded. 1951), and proteins in milk/mammary secretions were characterized by polyacrylamide gel electrophoresis (PAGE, Davis 1964), and they were determined before and after inoculation of Mycoplasma. All the organs including udders and mammary lymph nodes, were examined grossly and microscopically. The udder tissues and mammary lymph nodes from both halves of each goat were cultured for Mycoplasma isolation as described by Ban erjee et al (1979)
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