Abstract
Peroxidase, polyphenol oxidase (PPO), phenolic compounds and total sugars (TS) were investigated during root formation in cuttings of Berberis vulgaris var. asperma (BVA) and Berberis thunbergii var. atropurpurea (BTA) treated with indole-3-butyric acid (IBA) and IBA+H2O2. Rooting was observed on BTA cuttings but not on BVA cuttings. The BTA cuttings treated with IBA and IBA+H2O2 showed higher rooting percentages, number of roots, and root length over the control. Those treated with IBA+H2O2 recorded the lowest peroxidase activity after planting. BTA cuttings treated with IBA+H2O2 showed the highest peroxidase activity at 50 d after planting; BVA cuttings under different treatments showed no significant difference for peroxidase activity at planting time or up to 80 d after planting. PPO activity for the BTA cuttings in the control treatment was lower than for other treatments during root formation. The cuttings in the IBA and IBA+H2O2 treatments showed increased PPO activity from 0 to 50 d after planting and a slight decrease in PPO activity from 60 to 80 d after planting. PPO activity for the BVA cuttings was significantly lower than for BTA during root formation. The BTA cuttings treated with IBA and IBA+H2O2 showed the highest phenolic compound content during root formation. The BVA cuttings displayed higher TS than BTA during the initial stage of root formation. A comparison of the anatomical structure of easy-to-root and difficult-to-root cuttings indicated that physical inhibitors did not affect the rooting capacity of BVA.
Highlights
More than 40 cultivars of Japanese barberry (Berberis thunbergii DC) are utilized as ornamental shrubs in landscaping because they are hardy, drought tolerant, easy to grow and generally attractive
Berberis thunbergii var. atropurpurea (BTA) cuttings treated with indole-3-butyric acid (IBA) + H2O2 showed the highest peroxidase activity at 50 d after planting; Berberis vulgaris var. asperma (BVA) cuttings under different treatments showed no significant difference for peroxidase activity at planting time or up to 80 d after planting
The present study investigated biochemical and anatomical changes during rooting of Berberis thunbergii var. atropurpurea and Berberis vulgaris var. asperma barberry species
Summary
More than 40 cultivars of Japanese barberry (Berberis thunbergii DC) are utilized as ornamental shrubs in landscaping because they are hardy, drought tolerant, easy to grow and generally attractive. They vary in leaf colour (green, purple, yellow or variegated) and plant habitat, and are propagated from seeds and cuttings (Lubell et al, 2008). Atropurpurea (BTA) has adapted to the drought conditions of Khorasan province in Iran. It is a deciduous shrub with purple leaves and yellow aggregate blooms (Lubell et al, 2008). In the last 20 years, BVA has become a major crop in the area, with about 6000
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