Abstract
Cotton is one of world’s most essential fiber crops having global significance. Several biotic and abiotic stresses are constraints in cotton production including the bollworm complex and sucking pests. Among sucking pests, the cotton leafhopper, Amrasca biguttula biguttula (Ishida) is a devastating pest causing both quantitative and qualitative losses. After the introduction of Bt cotton, there was a check to the bollworm complex. But, the sucking pest complex particularly leafhoppers increased gradually reaching economic injury levels. The rampant application of insecticides to control sucking pests has led to the development of pest resistance to insecticides with different mode of action world over. Among the various mechanisms of resistance, the metabolic resistance plays a vital role. In order to determine the enzyme activity viz., cytochrome P450 monooxygenase, carboxyl esterase and glutathione S-transferase, for which the biochemical analysis was done by using field collected and laboratory reared population of this pest. The results revealed that, the activity of detoxification enzymes was significantly higher in the field collected population as compared to the laboratory population. The specific activity of cytochrome P450 monooxygenase (14.88 nmol/min/mg protein), carboxyl esterase (1321.97 nmol/min/mg protein) and glutathione S-transferase (1021.83 nmol/min/mg protein) in field collected population as compared to cytochrome P450 monooxygenase (12.02 nmol/min/mg protein), carboxyl esterase (1022.82 nmol/min/mg protein) and glutathione S-transferase (310.26 nmol/min/mg protein) in susceptible laboratory reared population of A. biguttula biguttula. This clearly shows that, these three xenobiotic enzymes are responsible for the detoxification of insecticides in the field population of this notorious pest.
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
