Abstract
A kinetically controlled microbial sensor characterized by a very low “microbial loading” with Bacillus subtilis has been developed. This sensor allows efficient measurement of the change of current after substrate addition. It is shown that this signal (indicating the acceleration of respiration) is connected with substrate uptake by B. subtilis. This conclusion is based on the results of experiments with glucose, α-methylglucose and glucose-6-phosphate as well as investigations on the influence of chloromercuribenzoate, dinitrophenol and sodium fluoride on the respiration after substrate addition. It is assumed that the measuring signal of the kinetically controlled microbial sensor reflects substrate uptake by the micro-organisms. Selectivity of the sensor was improved by induction or blocking of desired or undesired transport systems. Selective determinations of maltose, peptide (glycyl—glycine) and glutamic acid were achieved using this approach.
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