Abstract
Biochemical techniques were used to investigate the development of hepatoxic effects caused by single exposure to crude oil (CO, 3.0 ml/kg bw) and/or ciprofloxacin (CFX, 200 mg/kg bw) in male albino rats. The results showed that hepatic P-450 1A content in crude oil exposed rats was induced after<br />1 day to 4 fold and reached maximum 7.6 fold after 2 days compared with control and then fell to the first day degree. CFX exposed rats significantly inhibit the hepatic P-450 1A content while in crude oil and CFX co-exposed group it induced but in lesser degree than that induced by crude oil alone. Inhibition of cytochrome P-450 1A has no effect on the activity of lipid peroxidation (LPO) while its activation latter was caused by elimination of the inhibitor from the body and delayed activation of cytochrome P-450 1A. Single exposure of both crude oil and CFX lead to destructive changes in liver cells and inhibition of CYP1A by ciprofloxacin could not protect against lipid peroxidation induced by crude oil.
Highlights
Kazakhstan has the second largest oil reserves among the former Soviet republics after Russia and the problem of pollution of the territory by crude oil is one of the main ecological problems of Kazakhstan [1,2,3]
Hepatic P450 of the forth group animals was significantly (p
Our results have shown that following intraperitoneal injection of crude oil (3 ml/kg bw) there was significant induction of hepatic P450 level from the first day and reached maximal value by second day compared with control and fell to the first day degree
Summary
Kazakhstan has the second largest oil reserves among the former Soviet republics after Russia and the problem of pollution of the territory by crude oil is one of the main ecological problems of Kazakhstan [1,2,3]. Crude oil derived from crude petroleum contains complex mixture of chemicals, varying widely in composition of hydrocarbons and hydrocarbon like chemicals [4, 5]. Crude oil contains some trace elements like vanadium, nickel, iron, aluminium, copper, and some heavy metals like lead and cadmium [6]. Many of compound of crude oil are highly toxic and cancer causing. The most hazardous components of crude oil are aromatic compounds as like benzene [7]. One of cause of toxicity of crude oil is the process of metabolism in which the metabolite of a compound is more toxic than the parent chemical. The first step in the metabolism of petroleum by vertebrates is oxidation, catalyzed by the cytochrome P-450
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