Abstract
Diclofenac, an anti-inflammatory drug, is often detected in natural waters in the ng/L to μg/L range, posing a threat to aquatic organisms. The study focused on the effects of diclofenac in a gastropod mollusk Radix balthica. A 72-h exposure to environmentally relevant concentrations of diclofenac caused deviations from the baseline activities of the studied enzymes in the digestive gland of snails. Acetylcholinesterase activity was induced by the end of exposure, with the most pronounced increase at 3 μg/L. Results on glutathione-S-transferase activity were nonuniform, and no significant variations were observed in thiobarbituric acid reactive substances concentrations, indicating that diclofenac did not cause oxidative stress in the digestive gland of R. balthica at 0.04–4 μg/L range. Diclofenac lowered the oxygen consumption rate in snails in a concentration-dependent manner. At concentrations ≥0.9 μg/L, animals attempted to switch aquatic respiration to breathing air to regulate their metabolic needs. The study showed that diclofenac at environmentally relevant concentrations affected the fitness of R. balthica.
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