Abstract
We evaluated the endpoint of caries removal with a novel fluorescence-controlled Er:YAG laser by biochemical analysis of dentin collagen and electron microscopy. For the biochemical analysis 210 carious and 60 caries-free teeth were treated by a fluorescence controlled Er:YAG laser (n=240) or by conventional drilling (n=30). A layer of dentin from the cavity floor was removed to determine collagen crosslinks hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) by HPLC. Thirty teeth were examined by SEM and TEM. Approximately 100% HP and LP were found in carious dentin, in contrast to 0.33% (HP) and 0.68% (LP) in sound dentin. Following caries removal by laser 0.84% (HP) and 1.26% (LP) and 1.56% (HP) and 2.48% (LP) (threshold level 5, respectively 10) were found. The biochemical and ultrastructural analysis demonstrated the suitability of the fluorescence controlled Er:YAG laser to remove irreversibly denatured collagen of carious dentin.
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