Abstract
ABSTRACT The effects of 24-epibrassinolide (24-epiBL 10−8 M) were investigated on antioxidant enzymes activity, lipid peroxidation, and genes expression in 10-day-old Linum usitatissimum L. seedlings subjected to 6%, 12%, and 18% polyethylene glycol (PEG). Water deficit significantly enhanced the activity of CAT, POD, and SOD enzymes as well as elevation in protein, proline, and MDA contents whereas decreased activity of APOX and NR in a concentration-dependent manner. 24-epiBL seed priming improved flax adaptability due to the induction of antioxidant enzymes activity and protein and proline contents with a reduction in MDA content and antioxidant capacity. Gene expression studies showed that Mn-SOD, POD1, POD3, ERF, and WRKY 40 transcript levels were generally upregulated. Application of 24-epiBL caused induction in mRNA abundance of Mn-SOD, POD3, KRP2, and WRKY 40 under at least one level of PEG imposition. In conclusion, 24-epiBL seed priming could be implemented in order to counteract consequences of water deficit in flax.
Highlights
Flax, an annual herb, is one of the industrial oil seed crops grown in temperate climates
Seedlings exposed to the polyethylene glycol (PEG) exhibited a significant decrease in APOX activity under 6% PEG (32%), 12% PEG (61%), and 18% PEG (76%) compared with the control group (Figure 1 (A))
BR + PEG (6%), BR + PEG (12%) and BR + PEG (18%) treated plants, respectively, compared to the PEG plants under the same PEG exposure. 24-epiBL seed priming increased (25%) the enzyme activity under the BR treatment compared to the control group
Summary
Flax (linseed), an annual herb, is one of the industrial oil seed crops grown in temperate climates. The seed oil of flax is enriched in α-linolenic acid (ALA) (18:3cisΔ9, 12, 15) which makes it useful for a variety of industrial products. The linseed oil is a good source of ALA in the human diet (Czemplik and Szopa 2009). Plants mitigate abiotic stress-mediated consequences via adaptive capacity, mostly governed by the efficiency of the antioxidant defense system. This system includes ROS scavenging enzymes (such as superoxide dismutase, SOD; catalase, CAT; peroxidase, POD; and ascorbate peroxidase, APOX) and non-enzymatic components such as proline (Vardhini and Anjum 2015)
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