Biochemical and histopathological alterations in freshwater fish, Labeo rohita (Hamilton, 1822) upon chronic exposure to a commonly used hopper insecticide, triflumezopyrim

Abstract

The present study has been carried out to see the long-term effects of triflumezopyrim in an Indian major carp, Labeo rohita. Fishes were exposed to sub-lethal concentrations triflumezopyrim insecticide, 1.41 ppm (Treatment 1), 3.27 ppm (Treatment 2) and 4.97 ppm (Treatment 3), respectively for 21 days. The liver, kidney, gills, muscle, and brain tissues of the fish were examined for physiological parameters and biochemical parameters such as catalase (CAT), superoxide dismutase (SOD), lactate dehydrogenase (LDH), malate dehydrogenase (MDH), alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), acetylcholinessterase (AChE), and hexokinase. After 21 days of exposure, the activity CAT, SOD, LDH, MDH and ALT got increased and a drop in the activity of total protein was found in all treatment groups in comparison to the control group. Long-term triflumezopyrim exposure increased ROS production, ultimately leading to oxidative cell damage and inhibiting the antioxidant capabilities of the fish tissues. Histopathological analysis showed alteration in different tissues structures of pesticide treated fishes. Fishes exposed to highest sublethal concentration of the pesticide showed higher damage rate. The present study demonstrated that chronic exposure of fish to different sublethal concentration of triflumezopyrim exerts detrimental effect on the organism.