Biochemical and histological alterations in the prawn Macrobrachium lamerrei following exposure to automobile discharge

Abstract

Polycyclic aromatic hydrocarbons (PAH), important constituents of petroleum products, are known to enter freshwater systems via industrial effluents. In the present study the possible toxicity of automobile effluent presumably containing PAH was demonstrated in the freshwater prawn Macrobrachium lamarrei. The prawns were exposed for 1, 8, 15 and 28 days to two sublethal concentrations (0.75% and 3.75%) of automobile effluent. A decrease was noted in the total protein content in haemolymph and hepatopancreas of prawns exposed for 28 days to both these sublethal concentrations. SDS-PAGE revealed that the effluent caused reduction in high molecular weight proteins (106-90 kD) of the haemolymph. The exposure to automobile effluent resulted in changes in the lamellar structure of the gills and shrinkage of hepatopancreatic cell types. Thus it is imperative to discard such wastes carefully ensuring that there is no entry into aquatic ecosystems.