Biochemical and functional association between CD8 and H-2 at the surface of a T cell clone

Abstract

In an attempt to define structures interacting with CD8 molecules during activation of CD8 + cells, immunoprecipitates of CD8 and Tcr-CD3 molecules from lysates of a surface-labeled CTL clone were analyzed. No proteins other than the known Tcr α/β and associated CD3 components were detected in either anti-Tcr or anti-CD3 immunoprecipitates, whether or not the CTL clone had been activated. However, anti-CD8 antibodies co-precipitated class I MHC heavy chain and associated β2-microglobulin in all conditions. The latter co-precipitation was shown to result from “ cis-type” interactions between CD8 and class I MHC proteins on the same cell and to involve a degree of selectivity, as class I MHC molecules were absent from immunoprecipitates of highly expressed cell surface molecules such as LFA-1. A further analysis of cell surface molecular distribution during antigen-dependent CTL-target cell interaction by double fluorescence-microscopy in non-activating conditions indicated that an increased density of CTL class I molecules was found in the CTL-target cell contact zone of most conjugates with redistributed CD8 molecules. A possible role for “ cis-type” class I MHC-CD8 interactions in the dynamics of CTL-target cell contacts is proposed.