Biochemical and cytogenetic analysis of different grades of glioma patients

Abstract

Background: Glioma, a neoplasm of neuroglial cells, is the most common type of brain tumor, constituting more than 50% of all brain tumors. Purpose: This report summarizes the current knowledge regarding the clinical utility of biochemical enzyme markers for both diagnostic and therapeutic purposes in different grades of glioma. Methods: Sixty patients with different grades of glioma include glioblastoma multiforme (n=20), Anaplastic astrocytoma (n=10), Oligodendroglioma (n=10), Ependymoma (n=10), Pilocytic astrocytoma (n=10) and patients with benign lesions (n=5) served as controls . Activity of antioxidants and marker enzymes were assayed in all grades followed by karyotyping . Results: Activities of antioxidant enzymes reduced significantly compared to controls except CAT, GST that showed marked increase in + accordance with malignancy. CK, Na-K ATPases, 5'-Nucleotidases showed marked increase in grade IV. Similarly, Mg2-ATPase, Ca2+ATPases showed significant increase in grade III. Conclusion: The clinical importance for classification and treatment of glioma, biochemical enzyme markers. The study of enzymes supported by related chromosomal changes is anticipated to provide better appreciation of biological properties in different grades of glioma. doi : 10.5214/ans.0972.7531.1017305 Competing interests - None, Source of Funding - None Received Date : 6 April 2010 Revised Date : 10 May 2010 Accepted Date : 29 May 2010