Biochemical and Culture-based Approaches to Identification in the Diagnostic Microbiology Laboratory

Abstract

<h3>ABSTRACT</h3> The late 1800s through the early 1900s saw the rapid development of growth media containing various substrates, that is, carbohydrates, to identify and differentiate microbes isolated from clinical specimens. However, during the 1950s, an evolution of diagnostic services occurred created by a growing population with access to health insurance and the subsequent requirement for quality health care. The utilization of miniaturized, multitest kits provided the first significant advancement of the biochemical, growth-based approach needed to handle the increased demand for clinical services. These testing kits provided reductions in labor and material costs associated with making, maintaining, inoculating, and reading tubed and plated media while also reducing the time required to identify microbial isolates. The trend to improve laboratory efficiency and quality continued with the incorporation of automation and computers throughout the 1970s. Automated systems greatly increased the testing capacity of laboratories by allowing the simultaneous determination of identification and antimicrobial susceptibilities from 1 inoculum and by further reducing the time to identification to hours instead of days. Within just the last 10–15 years, development and integration of a new growth-based approach for identification have occurred in the form of matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The mass spectrometric approach provides the lowest cost per analysis and fastest time to identification after isolation of any current technique available in the microbiology laboratory. As health care costs and demand continue to increase and more hospitals look to consolidate laboratories, fully automated facilities incorporating mass spectrometry for identification, along with molecular methods, will become commonplace.