Biochemical and Biological Study on The Effect of Flaxseed on Rats Suffer from Nephropathy.

Abstract

The present study was conducted to investigate the effect supplemented diets with different doses of flaxseed on some biological, biochemical and histopathology changes in rats suffering from nephropathy. Thirty adult male albino rats Sprague Dawley strain weighing (170-175g) were divided into two main groups, the first main group fed on basal diet as a (negative control group). The second main group (24 rats) injection with 10 ml glycerol /kg dissolve in saline solution (glycerol / saline solution v/v) to induce renal failure, the dose of glycerol were injected under slight diethyl ether anesthesia. The second main group divided into four subgroups, the first subgroup fed standard diet (positive control group). The second subgroup fed standard diet containing 3% flaxseed. The third subgroup fed standard diet containing 5% flaxseed. The forth subgroup fed standard diet containing 7% flaxseed. At the end of the experiment (4 weeks), blood samples were taken; serum was separated and subjected to biochemical analysis (renal function). Glutathione, Malondialdehyde and histopathology were exanimated in kidney tissue in all groups. The results indicated that all treated groups get better in body weight gain%, Kidney functions (urea, uric acid and creatinine in serum) and GSH and MDA renal tissue ameliorated especially in renal failure rats fed on diet containing 5% and 7% flaxseed.