Biochemical Analysis of Nonhistone Protein Scaffold in Metaphase Chromosome of Common Wheat Triticum aestivum.

Abstract

Removing the histone and most of the nonhistone from mitotic chromosomes, we studied the protein composition of the chromosomal nonhistone protein scaffold extracted and separated from isolated chromosomes by using one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). We found that the scaffold in the metaphase chromosome of Triticum aestivum consists of 16 nonhistone species. The molecular weights of these nonhistone protein range from 18KD to 90KD; the major nonhistone protein with the molecular weight of 30KD, 32KD, 37KD, 43KD, 44KD, 50KD, 53KD, 63KD, 78KD and 88KD which can be clearly recognized in the gel are abundant in the scaffold. Our findings demonstrated the existence of nonhistone proteins scaffold in metaphase chromosome of common wheat. This scaffold seems to be similar to the scaffold found in the metaphase chromosomes of humans and animals, in terms of SDS-PAGE profiles.