Biochemical analysis by SDS-PAGE and Western blotting of the antigenic relationship between Leptospira and equine ocular tissues

Abstract

The antigenic relationship between Leptospira interrogans, equine cornea and lens was previously noted in our studies. Serum antibodies from horses inoculated with serovars wolffi, pomona, icterohaemorrhagiae, and tarassovi, were able to bind to five antigenic fractions from both cornea and lens, as demonstrated by immunoblotting. These antigens seem to be made up of protein and carbohydrates. After treatment with periodate for cleavage of glycoside ring structures, those fractions kept their condition of target for anti- Leptospira antibodies. Nevertheless, all fractions lost that condition after being subjected to peptide hydrolysis.