Biochar-induced variations in crop yield are closely associated with the abundance and diversity of keystone species

Abstract

Biochar application has widely been used to improve crop yield, but its effectiveness is uncertain. Soil microbial communities may play critical roles, but we lack experimental evidences on the relationships between these communities and crop yield following biochar application. Here, we used cooccurrence networks to demonstrate the importance of ecological clusters (cooccurring taxa of soil microbes including bacteria and fungi) and to identify specific keystone species that were closely connected with the variations in crop yield in a pot experiment. The experiment included two soils (i.e., red soil and yellow-cinnamon soil) for wheat growth, with each soil receiving three biochar application rate. The grain yield in the red soil significantly increased while that in the yellow-cinnamon soil significantly decreased with the biochar application rate. Generally, the grain yield from the two soils showed close correlations with the relative abundance as well as with the diversity of keystone species within major clusters rather than with the soil properties and enzyme activities. This contrasting effectiveness was mainly associated with the enrichment of beneficial and suppression of detrimental keystone species in the red soil and the suppression of beneficial and enrichment of detrimental keystone species in the yellow-cinnamon soil. These species together mainly accounted for the variation in the relative abundance of keystone species within major clusters of each soil, suggesting their potential to affect crop yield following biochar application.