Abstract

In order to explore a resource-saving and environmentally friendly approach to reduce nitrogen fertilizer and increase soil carbon pool in crop production. There are 5 treatments in the experiment namely CK: conventional fertilization and irrigation methods (pure nitrogen 30 kg/hm2); T1: drip irrigation fertilization method (pure nitrogen 15 kg/hm2); T2: drip irrigation fertilization method (pure nitrogen 15 kg /hm2), biochar-based fertilizer 600 kg/hm2; T3: drip irrigation method (pure nitrogen 15 kg/hm2), biochar-based fertilizer 1200 kg/hm2; T4: drip irrigation method (pure nitrogen 15 kg/hm2), biochar-based fertilizer is 1800 kg/hm2. The results showed that the microbial carbon content of the biochar-based fertilizer treatment under the condition of drip irrigation to reduce nitrogen was increased by 39.9%-68.4% as compared with the control. All treatments of the biochar-based fertilizer can significantly increase the soil easily oxidizable carbon in the early and mid-term growth. The content of solid carbon and soluble organic carbon increased by 31.3%-148.31% and 12.2%-63.92%, respectively. The application of biochar-based fertilizers significantly increased the net photosynthetic rate and stomatal conductance in the early and mid-transplanting period, as well as the transpiration rate and intercellular CO2 concentration throughout the growth period, and the SPAD, NDVI value, and dry matter accumulation. Under the condition of reducing nitrogen by 50%, drip irrigation combined with biochar-based fertilizer can significantly increase the soil microbial biomass carbon content, oxidizable carbon and soluble carbon content, promote the photosynthetic effect and dry matter accumulation of tobacco plants, and it is beneficial to promote crop planting.

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