Biochar amendments combined with organic fertilizer improve maize productivity and mitigate nutrient loss by regulating the C–N–P stoichiometry of soil, microbiome, and enzymes

Abstract

Coupled amendments of biochar and organic fertilizers may be one of the effective practice to ensure high cropland productivity and resource use efficiency, but there is little field-based evidence for this. Herein, we employed a eight-years (2014–2021) field experiment to explore the effectiveness of biochar and organic fertilizer amendments on crop productivity and nutrient runoff losses, as well as to further explored their relationships with the carbon:nitrogen:phosphorus (C:N:P) stoichiometry of soil, microbiome, and enzymes. Experiment treatments include No fertilizer (CK), chemical-only fertilizer (CF), CF + biochar (CF + B), 20% chemical N was replaced by organic fertilizer (OF), and OF + biochar (OF + B). Compared with the CF, the CF + B, OF, and OF + B treatments increased average yield by 11.5%, 13.2%, and 32%, average N use efficiency by 37.2%, 58.6%, and 81.4%, average P use efficiency by 44.8%, 55.1%, and 118.6%, average plant N uptake by 19.7%, 35.6%, and 44.3%, as well as average plant P uptake by 18.4%, 23.1%, and 44.3%, respectively (p ≤ 0.05). Compared with the CF, the CF + B, OF, and OF + B decreased average average total N losses by 65.2%, 97.4%, and 241.2%, and average total P losses by 52.9%, 77.1%, and 119.7%, respectively (p ≤ 0.05). Organic-amended treatments (CF + B, OF, and OF + B) significantly changed soil total and available C, N, and P content, soil microbial C, N, and P content, as well as the potential activities of soil C-, N-, and P-acquiring enzymes. Plant P uptake and P-acquiring enzyme activity were the main drivers of maize yield, which was influenced by the contents and stoichiometric ratios of soil available C, N, and P. These findings suggest that organic fertilizer applications combined with biochar have the potential to maintain high crop yields while reducing nutrient losses by regulating the stoichiometric balance of soil available C and nutrients.