Abstract
Lung cancer is among the most common malignancies with a poor 5-year survival rate reaching only 16%. Thus, new effective treatment modalities and drugs are urgently needed for the treatment of this malignancy. In this study, we conducted the first investigation of the effects of Biochanin A on lung cancer and revealed the mechanisms underlying its potential anticancer effects. Biochanin A decreased cell viability in a time-dependent and dose-dependent manner and suppressed colony formation in A549 and 95D cells. In addition, Biochanin A induced S phase arrest and apoptosis and decreased mitochondrial membrane potential (ΔΨm) in A549 and 95D cells in a dose-dependent manner. Our results of subcutaneous xenograft models showed that the growth of Biochanin A group was significantly inhibited compared with that of control groups. Finally, P21, Caspase-3, and Bcl-2 were activated in Biochanin A-treated cells and Biochanin A-treated xenografts which also demonstrated that Biochanin A induced cell cycle arrest and apoptosis in lung cancer cells by regulating expression of cell cycle-related proteins and apoptosis-related proteins. In conclusion, this study suggests that Biochanin A inhibits the proliferation of lung cancer cells and induces cell cycle arrest and apoptosis mainly by regulating cell cycle-related protein expression and activating the Bcl-2 and Caspase-3 pathways, thus suggesting that Biochanin A may be a promising drug to treat lung cancer.
Highlights
Lung cancer, known as primary bronchogenic carcinoma, is one of the most common malignancies
We investigated the cell viability, colony formation, mitochondrial membrane potential (ΔΨm), and expression level of cell cycle-related proteins and apoptosisrelated proteins with the lung cancer cell line A549, 95D, and the subcutaneous xenograft models to explore the underlying mechanisms and molecular signaling pathways by which Biochanin A blocks the cell cycle and induces apoptosis
We investigated the effect of Biochanin A on cell cycle arrest and apoptosis in lung cancer cells
Summary
Known as primary bronchogenic carcinoma, is one of the most common malignancies. Lung cancer has become the most serious of the malignant tumors and threatens the safety and the quality of human life worldwide. Research on the etiology, prevention, diagnosis, and treatment of lung cancer has become an important issue [1,2,3]. The limited progress in the treatment of lung cancer leads to a poor prognosis. The 5-year survival rate of lung cancer is only 16% since diagnosed. Research on the treatment of lung cancer and the development of new drugs are important for the prevention and cure of lung cancer. Research based on Chinese herbal medicine, especially based on a single ingredient, in the treatment of lung cancer is rare
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