Abstract
Polymer membrane surfaces have been equipped with the digestive enzyme trypsin. Enzyme immobilization was performed by electron beam irradiation in aqueous media within a one-step method. Using this method, trypsin was covalently and side-unspecific attached to the membrane surface. Thus, the use of preceding polymer functionalization and the use of toxic solvents or reagents can be avoided. The resulting membranes showed significantly improved antifouling properties as demonstrated by repeated filtration of protein solutions. Furthermore, the biocatalytic membrane can be simply “switched on” to actively degrade a fouling layer on the membrane surface and regain the initial permeability. The membrane pore structure (pore size and porosity) was neither damaged by the electron beam treatment nor blocked by the enzyme loading, ensuring a stable membrane performance.
Highlights
Porous polymer membranes are of increasing importance regarding modern separation technologies such as waste water treatment, sterilization filtration, hemodialysis, production of fine chemicals, dairy industry, etc. [1]
To comply with required process conditions these polymer membranes are predominantly fabricated from synthetic materials such as polyethersulfone (PES), polysulfone (PSf), or polyvinylidene fluoride (PVDF) [2]
Trypsin was successfully immobilized via electron beam irradiation on different polymer membranes
Summary
Porous polymer membranes are of increasing importance regarding modern separation technologies such as waste water treatment, sterilization filtration, hemodialysis, production of fine chemicals, dairy industry, etc. [1]. Porous polymer membranes are of increasing importance regarding modern separation technologies such as waste water treatment, sterilization filtration, hemodialysis, production of fine chemicals, dairy industry, etc. Membranes have to be regularly cleaned to remove the fouling layer, depending on the membranes have to be regularly cleaned to remove the fouling layer, depending on the filtration application. This may include a daily chemically enhanced backwash, a weekly maintenance filtration application. Used cleaning agents are strong oxidants such as hypochlorite and citric acid
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