Abstract
A new esterase-producing strain (Bacillus amyloliquefaciens WZZ002) that exhibits high hydrolytic activity, excellent enantioselectivity, and high substrate tolerance on Boc-dl-Alanine methyl ester was isolated from soil samples. The reaction temperature, pH, and neutralizer optima of the cell-mediated biocatalysis were 35°C, pH8.0, and NH3·H2O, respectively. The optimal substrate concentration was 2M, with a biocatalyst loading of 50g/L. Results showed that the enantiomeric excess values of substrate and product were both greater than 99%. Thus, bioprocessing with the use of the isolated strain is a promising route for the commercial production of Boc-d-Ala-OMe.
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