Abstract
The present study is focused on dextranase from Streptomyces sp. NK458 with potential to remove dextran formed during sugar manufacture. The dextranase had molecular weight of 130kDa and hydrolyzed 15–25 and 410kDa dextran. Dextranase production was optimized using statistical designs and the enzyme was purified 1.8-fold with 55.5% recovery. It displayed maximum activity at pH 9.0 and 60°C and was stable over a wide range of pH from 5.0 to 10.0. The km and Vmax values were 3.05mM and 17.97mmol/ml/h, respectively. Ten units of dextranase could reduce dextran content by 67% in 24h and 56% in 72h from sugarcane juice of cane variety CoS 86032. The enzyme was stable up to 3days at 30°C beyond which its activity decreased and dextran removal could be retained by supplementation of 5U of dextranase. These properties make it a promising biocatalyst for sugar industry.
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