Abstract
The dried mango fruit processing industrial waste (MIW) used as carbon source for the production of pectinase from fungal strains. Eight fungal strains were isolated from MIW and screened for their ability to produce pectinase by pectin clear zone (PCZ) technique. Fusarium sp. (PSTF1) showed highest PCZ value of 52 mm. The physico-chemical characteristics of the medium were standardized for high production of pectinase. The highest production of pectinase in submerged fermentation observed at temperature—28 °C, pH-6.0, inoculum-size 0.6/25 ml, incubation—72 h, substrate concentration—0.6 g/100 ml, carbon source-fructose (1 %). The effect of different amino acids, vitamins also observed. Under these optimal conditions the highest activity 81.9718 µmol/ml of pectinase was observed. The Fusarium sp. (PSTF1) has been considered as the best pectinase producer in submerged fermentation of MIW. The cheap waste raw material used as best carbon source for high production of high value pectinase.
Highlights
Mango is an important tropical fruit crop of semi-arid countries including India
Apart from these, apple pomace, orange waste and other fruit and vegetable industrial wastes and different agro-industrial wastes were used for the production of pectinase, cellulase, a amylase and esterase and peroxidase are being in much use (Smith and Aidoo 1988; Attyia and Ashour 2002)
The isolated fungal strains identified as Fusarium sp. (PSTF1), Lichtheimia sp. (PSTF2), Pseudolagarobasidium sp. (PSTF3), Pencillium sp. (PSTF4), Cladosporium sp. (PSTF5), Fusarium sp. (PSTF6), Fusarium sp. (PSTF7), Fusarium sp. (PSTF8)
Summary
Mango is an important tropical fruit crop of semi-arid countries including India. More than 90 countries all over the world are producing mango fruits. Mango fruit processing industries are dumping waste on open fields and usually this waste is used as cattle feed, fuel wood or deposited as landfills (Devendra et al 2012) This leads to serious environmental pollution, as well as adversely effecting on livestock leading to death due to feeding of this contaminated waste (Andhra Jyothi News Paper, Chittoor, 2012). Aspergillus, Penicillium, Trichoderma, Mucor and Yarrowia etc., (Pariza and Foster 1983; Mudgett 1986) Fungi can produce both intracellular as well as extracellular enzymes but for breaking down of larger complex compounds like pectin, fungi secrete extra cellular enzymes (pectinases). (PSTF1) in submerged fermentation for the conversion complex mango industrial waste into simpler degraded products (humus) as it has shown highest pectinolytic activity when compare with other fungal isolates The present investigation was undertaken to produce pectinase from the isolated fungal strain Fusarium sp. (PSTF1) in submerged fermentation for the conversion complex mango industrial waste into simpler degraded products (humus) as it has shown highest pectinolytic activity when compare with other fungal isolates
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
