Abstract
Apart from natural CO2 sequestration, biocalcification process can reduce bioavailability of certain pollutants. In the present study, experiments were conducted to evaluate the change in bioavailability for three polycyclic aromatic hydrocarbons (PAHs) — anthracene (Ant), phenanthrene (Phe) and pyrene (Pyr) — either in normal soil extract that was spiked with PAHs (SESP) or spiked soil, after chemical calcification or biocalcification process. Results suggested that PAHs extractability in SESP (Ant: 2–4 mg/l, Phe: 3–6 mg/l and Pyr: 4.5–9 mg/l) after chemical precipitation of carbonate were not statistically different when compared to negative controls. However in biocalcification experiment the difference was statistically significant when conducted with SESP (P = 0.0425, α = 0.10) or soil (P = 0.035, α = 0.10). Supporting experiment revealed that the presence of microbial cells and sequence of Ca2+, CO3−2 addition was important in determining the extent in PAHs bioavailability reduction.
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