Abstract

Abstract: This research aimed to study biobutanol production using Lignocellulosic renewable substrate Rice Husk, carried out using Clostridium acetobutylicum MTCC 11274 via the Separate hydrolysis and fermentation (SHF) process. The simple sugars were exposed using a delignification process with different concentrations of Ammonia solution and some were hydrolyzed using conc. H2SO4 after getting treated with ammonia. The sugars were estimated using DNSA Method after which samples were fermented. The concentration of Biobutanol was estimated using GC-MS showing 2.2 gm/L in one of the tested samples.

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