Bioavailability of zinc and phosphorus in calcareous soils as affected by citrate exudation

Andreas Duffner,Ellis Hoffland,Erwin J M Temminghoff

doi:10.1007/s11104-012-1273-9

Abstract

Aims Zinc (Zn) and phosphorus (P) deficiency often occurs at the same time and limits crop production in many soils. It has been suggested that citrate root exudation is a response of plants to both deficiencies. We used white lupin (Lupinus albus L.) as a model plant to clarify if citrate exuded by roots could increase the bioavailability of Zn and P in calcareous soils. Methods White lupin was grown in nutrient solution and in two calcareous soils in a rhizobox. Rhizosphere soil solution was sampled to determine citrate, metals and P. Based on the measured citrate concentrations, a soil extraction experiment with citrate as extractant was done. Results Absence of Zn triggered neither cluster root formation nor citrate exudation of white lupin grown in nutrient solution, whereas low P supply did. The maximum citrate concentration (∼1.5 mM) found in the cluster rhizosphere soil solution of one soil mobilized P, but not Zn. In the other soil the highest citrate concentration (∼0.5 mM) mobilized both elements. Conclusions White lupin does not respond to low Zn bioavailability by increasing citrate exudation. Such a response was observed at low P supply only. Whether Zn and P can be mobilized by citrate is soil-dependent and the possible controlling mechanisms are discussed.

Highlights

Zinc (Zn) deficiency is limiting crop production in ±30 % of the world’s soils (Sillanpää and Vlek 1985)
With a nutrient solution experiment we studied the formation of cluster roots and the exudation of citrate by white lupin in absence of Zn and P
Cluster root formation was observed neither on plants grown in the nutrient solution without Zn nor on those grown in the complete nutrient solution

Summary

Introduction

Zinc (Zn) deficiency is limiting crop production in ±30 % of the world’s soils (Sillanpää and Vlek 1985). In these areas, Zn deficiency is often caused not by low total soil Zn contents but by low bioavailability of Zn (Alloway 2009). Quijano-Guerta et al (2002) studied the tolerance of rice (Oryza sativa L.) germplasm to Zn deficiency. They showed that genotypes tolerant to Zn deficiency have at least a moderate tolerance to P deficiency. They showed that genotypes tolerant to Zn deficiency have at least a moderate tolerance to P deficiency. Hoffland et al (2006) related these characteristics of multiple tolerance to citrate exudation

Methods

Results

Conclusion